---
title: FastGene Double Digest - One Reaction, Two Cuts!
description: Our FastGene Double Digest Chart helps you to quickly find the perfect conditions for DNA cutting with TWO Restriction Enzymes.
image: https://news.nippongenetics.eu/hubfs/shutterstock_5013046_pink_small.jpg
---
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# FastGene Double Digest - One Reaction, Two Cuts!
Published by [Jakob Maciejko](https://news.nippongenetics.eu/en/author/jakob-maciejko) on May 12, 2021, 1:54:45 PM
Our FastGene® Double Digest Chart helps you to quickly find the perfect conditions for DNA cutting with TWO Restriction Enzymes.
---
#### FastGene® Restriction Enzymes - Excellent performance.
Our FastGene® Restriction Enzymes are excellent DNA cutting tools. They show highest activity, reliability and purity, giving best results for scientists!
Setting up a reaction is very simple using the color coded buffer system. What's even simpler: Almost all FastGene Restriction Enzymes are compatible with our FastCut buffer - complete your reaction in 15 min!
#### Switch Made Easy.
There is no reason not to switch! In case you were using competitor N Enzymes before, FastGene® Restriction Enzymes are 100% compatible with all competitor N buffers.
#### Double Digest - Give your DNA directionality.
Double Digest can help you with your cloning experiments. The simultanous DNA cutting with TWO restriction enzymes is frequently used as a powerful cloning tool:
- - - "Sticky Ends"-restriction enzymes leave behind a specific overhang sequence, giving your DNA fragments directionality.
- When a DNA backbone and DNA insert are digested with the same pair of restriction enzymes (e.g. BamHI and EcoRI) only the cohesive "sticky-ends" can join together.
- This allows full control over the direction of DNA fragment alignment.
- The gaps are then closed by ligation, creating your final DNA fragment.
#### 
#### Double? No Trouble! - How to find the best Double Digest conditions.
Finding the best Double Digest conditions using TWO enzymes IN ONE reaction is no trouble at all. We prepared a chart for you (excerpt is shown below). All you need to do is pick your FastGene® Restriction Enzyme pair and the chart will display the recommended buffers for the reaction.
One buffer to cut them all! - 96% of our restriction enzymes can be used with our FastCut® buffer with 100% activity. There is no other way for faster and easier Double Digestion than with FastCut®.
Get the complete Double Digest chart for 115 FastGene® Restriction Enzymes:
[](https://hubspot-cta-redirect-eu1-prod.s3.amazonaws.com/cta/redirect/8262428/5a0c839a-db8b-446c-9f51-f1a23d339cd5)
---
#### Want to try Double Digest?
We understand that, as a scientist, you would like to test our FastGene® Restriction Enzymes before purchasing them. Just contact us by entering your details and we will organize the testing with us or one of our distributors.
Tags: [Cloning](https://news.nippongenetics.eu/en/tag/cloning), [Applications](https://news.nippongenetics.eu/en/tag/applications)
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