---
title: FastGene Double Digest - One Reaction, Two Cuts!
description: Our FastGene Double Digest Chart helps you to quickly find the perfect conditions for DNA cutting with TWO Restriction Enzymes.
---

[NIPPON Genetics EUROPE News](https://news.nippongenetics.eu/en)

# [FastGene Double Digest - One Reaction, Two Cuts!](https://news.nippongenetics.eu/en/fastgene_double_digest)

 Written by [Jakob Maciejko](https://news.nippongenetics.eu/en/author/jakob-maciejko) | May 12, 2021 11:54:45 AM

Our FastGene® Double Digest Chart helps you to quickly find the perfect conditions for DNA cutting with TWO Restriction Enzymes.

#### FastGene® Restriction Enzymes - Excellent performance.

Our FastGene® Restriction Enzymes are excellent DNA cutting tools. They show highest activity, reliability and purity, giving best results for scientists!

Setting up a reaction is very simple using the color coded buffer system. What's even simpler: Almost all FastGene Restriction Enzymes are compatible with our FastCut buffer - complete your reaction in 15 min!

 

#### Switch Made Easy.

There is no reason not to switch! In case you were using competitor N Enzymes before, FastGene® Restriction Enzymes are 100% compatible with all competitor N buffers.

 

#### Double Digest - Give your DNA directionality.

Double Digest can help you with your cloning experiments. The simultanous DNA cutting with TWO restriction enzymes is frequently used as a powerful cloning tool:

- - - "Sticky Ends"-restriction enzymes leave behind a specific overhang sequence, giving your DNA fragments directionality.
          - When a DNA backbone and DNA insert are digested with the same pair of restriction enzymes (e.g. BamHI and EcoRI) only the cohesive "sticky-ends" can join together.
          - This allows full control over the direction of DNA fragment alignment.
          - The gaps are then closed by ligation, creating your final DNA fragment.

#### Double? No Trouble! - How to find the best Double Digest conditions.

Finding the best Double Digest conditions using TWO enzymes IN ONE reaction is no trouble at all. We prepared a chart for you (excerpt is shown below). All you need to do is pick your FastGene® Restriction Enzyme pair and the chart will display the recommended buffers for the reaction.

One buffer to cut them all! - 96% of our restriction enzymes can be used with our FastCut® buffer with 100% activity. There is no other way for faster and easier Double Digestion than with FastCut®.

Get the complete Double Digest chart for 115 FastGene® Restriction Enzymes:

#### Want to try Double Digest?

We understand that, as a scientist, you would like to test our FastGene® Restriction Enzymes before purchasing them. Just contact us by entering your details and we will organize the testing with us or one of our distributors.

[View full post](https://news.nippongenetics.eu/en/fastgene_double_digest)

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