FastGene® RNA Premium Kit: unraveling TRBP role in apoptosis

Written by Mariya Hrynchak | Sep 10, 2024 9:40:34 AM

In a recent study published in Nucleic Acids Research, researchers employed the FastGene^® RNA Premium Kit to investigate the intricate role of TRBP (TAR RNA-binding protein) in apoptosis during viral infection. This newsletter delves into the specifics of how this kit was instrumental in their research.

Purpose

The study specifically focuses on the interplay between RNA, protein, and viral infection. The primary objective was to understand the mechanisms by which TRBP influences cell death (apoptosis) in response to viral challenges. To achieve this, the researchers sought to identify microRNAs (miRNAs) associated with TRBP and explore their potential involvement in apoptosis regulation.

RNA extraction with FastGene^® RNA Premium Kit

The FastGene® RNA Premium Kit proved to be a crucial tool in this research. It was employed to extract high-quality total RNA from human HeLa TRBP−/− cells transfected with plasmid encoding TRBP-WT, -N or -D234A, following Sendai virus infection.

This kit is renowned for its efficiency in removing contaminants, including genomic DNA, which is essential for downstream applications like RT-qPCR (quantitative reverse transcription PCR).

Downstream Applications

The high-quality RNA extracted using the FastGene^® RNA Premium Kit from the specified HeLa cells was crucial for the study's success. The isolated RNA was used to study gene expression on a large scale through microarray analysis and confirm the previous data described in the paper through quantitative reverse transcription (qRT)-PCR, thus enhancing the overall robustness of the research findings.

Conclusion

The FastGene® RNA Premium Kit played a pivotal role in the successful execution of this research. By providing high-quality RNA from the specified HeLa cells, the kit enabled the researchers to accurately investigate the complex relationship between TRBP and apoptosis during viral infection. This study highlights the kit's utility as a valuable asset for researchers in various fields of molecular biology.

The full article: https://academic.oup.com/nar/article/52/9/5209/7643295