Our MIDORIGreen variants give perfect DNA signals and were designed for different light sources. All DNA-dyes are completely safe to use without any toxic or carcinogenic properties.
Overview of the applied staining methods and optimal excitation sources for our MIDORIGreen variants.
MIDORIGreen Advance is optimized for use with UV-light transilluminators. Compared to ethidium bromide (EtBr), MIDORIGreen Advance is non-toxic and non-carcinogenic but delivers very strong signals of DNA/RNA with an excellent signal-to-background ratio. These properties will make it a perfect replacement for EtBr, if UV-light is used as the excitation source. The DNA-stain is applied for in gel staining, when casting agarose gels, or post staining, after the electrophoresis run.
MIDORIGreen Xtra gives excellent results when used with Blue/Green-LED Technology or Blue-LED light. It is a highly sensitive stain, emitting very strong signals with low background fluorescence, making the detection of smallest DNA/RNA quantities very easy. With Blue/Green LEDs, MIDORIGreen Xtra delivers even better results than EtBr, reaching new levels of sensitivity. Its safe, non-toxic properties were comfirmed by independent laboratories. It can be used for in gel staining, when casting agarose gels, or post staining, after the electrophoresis run.
As its name suggests, MIDORIGreen Direct, is directly added to your DNA/RNA sample for visualization in an agarose gel. The direct staining of the sample eliminates staining of the gel background, providing perfect signals and an excellent signal-to-background ratio. MIDORIGreen Direct was developed and optimized for use with Blue/Green-LED or Blue-LED light. However, signals can also be detected with a regular UV-light transilluminator. Its safe, non-toxic properties were confirmed by independent laboratories. The 10x sample loading buffer is integrated in MIDORIGreen Direct, so samples can be loaded onto the agarose gel, subsequently after staining.
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Safe to useProven to be non-carcinogenic and non-toxic dyes for DNA/RNA detection in agarose gels, the MIDORIGreen family is a safe alternative to the traditional nucleic acid stain ethidium bromide. |
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Perfect SignalsThe chemical structures of our MIDORIGreen dyes have been optimized for an optimal absorption peak when bound to DNA. The dyes deliver strong signals with an excellent signal-to-background ratio. |
Affordable PriceThe MIDORIGreen dyes are available in a highly concentrated solution (25,000 x). The overall costs per gel is 3-4 x lower when compared to other DNA binding dyes. |
For all common light sourcesThe MIDORIGreen variants were developed for different light sources and flexible usage. MIDORIGreen Advance is optimal for UV-light, while MIDORIGreen Xtra and MIDORIGreen Direct give perfect results with Blue/Green or Blue LEDs. |