Library preparation is the most critical step in the NGS (next generation sequencing) workflow. The preparation of DNA fragments of specific size ranges is the basis of high-quality analyses. Therefore, monitoring the sample size and quality of the NGS library is of paramount.
The Qsep100 analyzer showed through e.g.,: easy use + innovative microcapillary electrophoresis technology with ready-to-use and replaceable gel cartridges + high resolution and high throughput + reduced cost of the instrument and also the costs per sample analysis.
Next-generation sequencing (NGS) is widely used in genomics, epigenetics and transcriptomics, etc.,1 and also in such clinical diagnostics applications as cancer screening and drug development.2 There are multiple commercial NGS platforms available, each incorporating unique operating principles (e.g., Roche 454FLX [454 Life Sciences, Branford, Conn.], Illumina Genome Sequencer3 [Illumina Inc., San Diego, Calif.] and Applied Biosystems/ABI SOLiD Sequencer [Foster City,Calif.]). No matter the platform, library preparation is the most critical step in the NGS workflow.
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