---
title: GreenGate - TypeIIS RE Cloning System for Plant Transgenesis
description: A new way to clone transgenic plants using TypeIIS restriction enzymes
image: https://news.nippongenetics.eu/hubfs/shutterstock_1482996404_biotechnologie-pflanzen.jpg
---
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# GreenGate - TypeIIS RE Cloning System for Plant Transgenesis
Published by [Dr. Marcelo Lanz](https://news.nippongenetics.eu/en/author/dr.-marcelo-lanz?hsLang=en) on May 14, 2021, 10:13:00 AM
Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J (2013) GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. PLoS ONE 8(12): e83043. [https://doi.org/10.1371/journal.pone.0083043](https://doi.org/10.1371/journal.pone.0083043)
The Golden Gate cloning method is an easy and straight forward procedure. Here, Lampropoulos et al. describe a variety of it designed for plant transgenesis, calling it cleverly GreenGate. The whole procedure is possible due to type IIS restriction enzymes. These enzymes cut at a defined number of nucleotides after the recognition site. Our FastGene BsaI is such an enzyme.
The plant transformation vectors created for this system include:
- Promoter
- N-terminal tag
- Coding sequence (CDS) (i.e. the gene of interest)
- C-terminal tag
- Plant terminator
- Plant resistance cassette
The major advantage of using GreenGate is a dramatic reduction of plasmid generation time when building a multi-construct transgenic plant.
Would you like to read the paper?
Just click on the link below.
[GreenGate Paper](https://doi.org/10.1371/journal.pone.0083043)
More information about the Bsa I Enzyme:
[FastGene Restriction Enzyme Bsa I](https://www.nippongenetics.eu/en/products/cloning/restriction-enyzme-finder/bsa-i/)
Tags: [Cloning](https://news.nippongenetics.eu/en/tag/cloning), [Applications](https://news.nippongenetics.eu/en/tag/applications)
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